Life Sciences

Background: Highly pathogenic Porcine epidemic diarrhea virus (PEDV) is causing damages to the swine industry and is responsible for loss of economics. Vaccination is the most effective method to prevent and control PEDV infections. 
Methods: Recombinant virus-like particles (VLPs) by baculovirus expression vector system have been suggested as a promising platform for new viral vaccines. 

Chlorpyrifos a broad spectrum product has been in use for many applications in home and agriculture against the damages caused by pests around the globe. This extreme usage has lead to detrimental effects on biological life. The goal of the present study was to determine the toxic effects of chlorpyrifos during early development in zebrafish. The embryo/larvae were exposed to 200μg/L, 400μg/L, 600μg/L, 800μg/L and 1000μg/L of CP and observations were made at 24hpf, 48hpf, 72hpf and 96hpf. Changes in the protein content was estimated and proteins were resolved by SDS-PAGE.

In this study, the anti-Oxacillin Resistant Staphylococcus aureus (ORSA) activity of extracts from Eugenia pyriformis (leaf and stem), Plinia cauliflora (rind fruit, leaf, and stem), and Heliconia rostrata (flower, rhizome, leaf, and stem) was evaluated. In vitro screening of antibacterial activity of the extracts (extraction with ethanol:water [7:3, v/v]) was performed using both agar diffusion and broth microdilution assays against sixty isolates of ORSA from dental clinic (genotyped by using Multilocus Enzyme Electrophoresis method) and against S. aureus reference strain (ATCC 6538).

The present study is to analyse the impact of diluted distillery effluent on the growth  of rice (Oryza sativa L.). Of the different concentrations of the effluent used, 20% dilution was found to be best suited for the plant growth. The results indicate that after proper dilution, distillery effluent can be used effectively as a source of fertilizer irrigant. This also can be considered as an effective effluent disposal option too.

Recent studies evaluated that immunoproteomic vaccines provide protection against bacterial infections in fish aquaculture and the vaccines are popular due to its long lasting immunity, safety and low cost versatile characteristics. In this study we used Whole cell – Formalin Killed (WC) and Outer Membrane Protein (OMP) of F. Columnare as a vaccine to provide protection against columnaris disease in fish Catla catla. We used the extract of Asparagus racemosus as an adjuvant in the vaccine preparation.

Surgical procedures are frequent these days, as well as the search for appropriate and safe procedures for patients, requiring more research and optimize nursing care. The correct procedures in the surgical dressing are also important to the success of the intervention.
Purpose: To identify the need for surgical dressing in postoperative.
Methods: A study has been carried out which consists in systematic literature revision and it has taken place where seven items were selected. 

Investigation was conducted to study the effect of different chemicals as a foliar spray on bitter gourd.  The treatments were comprised of plant growth regulators viz., NAA @ 50 mg/l and 75 mg/l, ethereal @ 50 mg/l and 100 mg/l, spermine @ 5 mg/l and 10 mg/l, putrescine @ 20 mg/l and 40 mg/l and Control (water spray) applied at 2 and 4 leaf stage. All the treatments improved the flowering and yield characters over control. However, among different treatments, foliar spray of NAA 75 mg/l followed by, putrescine @ 40 mg/l were found most significant in influencing parameters like no.

Lemongrassis an important aromatic, anti-microbial crop that checks the viability of tumour cell. Citral is its active constituent that is used in preparation of ionones and artificial perfumes. Keeping this in mind, the importance of crop, a pot experiment was conducted as a factorial randomized design to find out the effect of basal application of polyacrylamide on lemongrass. Polyacrylamide is a long chain, synthetic and a high molecular weight polymer. Treatments include six basal levels of polyacrylamide viz.Pam0,Pam224, Pam448, Pam896, Pam1792and Pam3584.

Objective: To evaluate in vitro, the synergistic effects  of bioactive extracts of Punica granatum (Pomegranate) "pulp", Actinidia deliciosa (Kiwifruit) "peel" and Citrus maxima (Pomelo) "pulp" with different antibiotics against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. Methods: The extraction was carried out using the soxhlet in presence of ethanol, methanol or water.  Evaluation of synergistic activity was carried out using disc diffusion method.

In the work, a study of mass fragmentation routes by the electron-impact mass spectrometry data has been examined for two open chain intermediates of benzimidazole derivatives and two imidazobenzodiazepines. By the isolation of open chain intermediate and the mass spectra, the structures of imidazobenzodiazepine have been confirmed.