Life Sciences

A good DNA extraction procedure for the isolation of genomic DNA should yield adequate and intact DNA of reasonable purity. The procedure should also be quick, simple, and cheap and if possible should avoid the use of dangerous chemicals. This paper describes a simple, rapid and efficient method for isolating genomic DNA extraction methods for mature leaves, resting buds and seedling leaves of 5 different genera namely Sapindus, Cardiospermum, Litchi, Allophilus, Dodonea of Sapindaceae.

Adolescent is a period whereas most thrilling, challenging, distressing and consequential aspects. They need to have a number of skills to function as individual and as a member of social group. The present study is undertaken to assess the role of life skills and social maturity among adolescence to get employment. The study was conducted in Tirupati town. Sample consists of 100 adolescence (50 boys) and (50 girls) were selected from five Government High schools by using purposive random sampling method.

In order to understand the photosynthetic physiological characteristics of Davidia involucrata, using portable LI-6400 photosynthesis Davidia involucrata measured net photosynthetic rate ( Pn), stomatal conductance (Gs), CO2 concentration between cells (Ci) and transpiration rate (Tr).

Character association and component analysis at genotypic level were studied in mutagenised populations (M2) of grasspea land race “Dhenkanal local” following seed treatment with gamma-rays, EMS, NG and their combinations. Seeds/plant emerged as an independent direct yield contributing trait in all treatments except 40kR gamma-rays + 0.2% EMS. In general, number of branches/plant, pods/plant and seeds/plant exhibited very high and significant positive association with seed yield/plant.

Many environmental factors, biotic and abiotic interact to influence the organismal development. Organisms respond to environmental stresses with behavioral, physiological or morphological adjustment to counter its effects and maintain normal functioning. In the present study, the high parental density results in offspring with smaller body size compared to offspring from parents that reproduced at lower densities.

A field experiment was conducted to study the effect of composted coirpith, composted pressmud, farmyard manure and NPK on soil enzyme activities and nutrient status of post harvested soil of clusterbean.

Thymus Capitatus is a shrub, which is native of the Mediterranean basin. The purpose of this study was to determine the chemical composition and inhibitory effect of essential oil of Thymus Capitatus on the corrosion of mild steel in hydrochloric acid (1M).The essential oil of Thymus Capitatus has been studied using gas chromatography (GC) and GC-mass spectrometry (GC-MS).

The present study was carried out to optimize cheese whey media composition for maximum lactic acid production by Lactobacillus casei using Response Surface Methodology. Four media components i.e. CSL, K2HPO4, MgSO4, and Sodium acetate were subjected for optimization Response surface methodology (RSM) a central composite design to determine the optimal concentration of these components for lactic acid production by Lactobacillus casei. A satisfactory fit of the model was realized. Lactic acid production was significantly affected by interaction of CSL, K2HPO4, MgSO4, and Sodium acetate.

Effect of 10 and 30 % sublethal concentration (12.4 ppm; 96 h LC50) of nickel on the haematology of Liza parsia after the exposure period of 30 days was studied. The RBC count (-35.82%) and Hb content (-63.28%) decreased significantly with an increase in WBC count (+28.95%). The light microscopic studies revealed many morphological changes in the RBC of fish such as wrinkling of cell membrane, vacuolization and rupture of erythrocytic membrane.

Effect of 10 % sublethal concentration (12.4 ppm; 96 h LC50) of nickel on the gill ultra structure of Liza parsia after the exposure period of 30 days was studied. The SEM studies revealed many morphological changes in the gill of Liza parsia such as epithelial hypertrophy, fusion of secondary lamellae, necrosis and degenerating microridges with mucous opening due to chronic exposure of the nickel.