Life Sciences

Optimization of a non-tissue method of agrobacterium mediated gene transfer in cowpea (vigna ungiculata l. walp.)

Agrobacterium infiltration is a common method used for effective gene transfer in many cultivated plants. In this study, the various concentrations (0.5, 1.0 and 1.5 × 109 cells ml-1) of A.tumefaciens was injected into the young leaves and germinated seeds of Cowpea by syringe method and vacuum infiltration. Then the morphological and biochemical variations were observed. The results show that the vacuum infiltration method was more efficient then the syringe infiltration.

Comparative assessment of field methods for microbiological water quality testing in emergencies

The combination of Comparative Analysis Performance and Decision Rank Matrix results were used to assess; accuracy, experimental process, cost per 1000 samples, portability and durability of Delagua, Colilert (P/A), Colilert (MPN) and Petrifilm methods for examination of microbiology water quality during emergencies. The assessment further compared human body incubation techniques with conventional power supplied incubation Colilert MPN is ranked (1st ) followed by Petrifilm (2nd) and lastly the Delagua (3rd) method.

Production and nutritional analysis of itsekiri pepper soup spices

The study was aimed at producing and analyzing the nutritional components of a typical It sekiri food seasoning (pepper soup spice). The following ingredients (1) Monodora Mystristica (Iwo), (2) Tetrapleura Tetreapera (Iyanghangangh), (3) Panirari Curatellifolia (Aghafilo), (4) Chrysobalanus Icaco and (5) Xylopia Ethiopica were bought, cleaned and ground, salt was also added to taste. Sample of the pepper soup spice (per 100g of sample) was analysed for its nutritional contents.

Assessment of watershed management impacts on erosion and sedimentation (behvard watershed, Iran)

The implementation of soil preservation programs and measures to combat erosion and sedimentation could substantially assist the protection of resources and the maintenance of the reservoir constructions under the framework of watershed management activities. The assessment of watershed management activities and the level of public support is essential in facilitating the enhancement of such steps and identifying their strengths and weaknesses. Thus, the objective of this research was to assess the impact of watershed management activities on erosion and sedimentation of Behvard Watershed.

Different insect pests in muga host plant som (persea bombycina) ecosystem

The Muga silk is one of the most precious silk produced by Antheraea assamensis is monopoly of Assam. The silk is very famous due to its unique golden yellow colour and lustre of the fibre. The Muga silkworm is present only in the North-Eastern region of India. This silkworm primarily feeds on Som plant (Persea bombycina) but some other plants like Litsea polyantha, Litsea salicifolia and Litsea citrate are used as host plant of the silkworm. The som plant (Persea bombycina), is an aromatic non deciduous tree with alternate leaves that are varying size and shape.

Soil erossion assessment in neyyar wildlife sanctuary using geoinformatics

Soil erosion is the process of detachment, transportation and deposition of soil particles from land surface. Agencies or the energy sources involved in the process of soil erosion are mainly water, wind, sea waves, human beings and animals. Soil erosion is a growing problem in Western Ghats of Kerala and particularly in the Neyyar wild life sanctuary in southern western Ghats, with rich biodiversity. Soil erosion not only decreases biodiversity of the area, but also reduces the water availability. In the current study, an effort to predict potential annual soil loss has been conducted.

Histological studies on the combined effect of synthetic pyrethroid lambda cyhalothrin and organophosphate chlorpyrifos on reproductive tissues in freshwater cultivable fish Cl

In the present study freshwater commercially cultivable catfish Clarias batrachus was used to evaluate the  combined  effect of  lambda cyhalothrin, a synthetic pyrethroid  and chlorpyrifos, an organophosphate insecticide widely used in agricultural fields. Clarias batrachus uses rice paddy slurries as its breeding and  nursery grounds, inhabit sediments as they are benthic  and are posed to greater threat from sediment associated toxic compounds such as pyrethroids and  organophosphates  mixtures which are  widely used in rice fields today to control rice pests.

Effect of plant growth regulators on in vitro shoot multiplication from callus of Manihot Esculenta Crantz CV. TMS 92/0326

The aim of this study was to test the effect of plants regulators on shoots multiplication in cassava cv TMS 92/0326 grown in liquid media with addition of 6-bezylaminopurine (BAP), and α-naphthaleneacetic acid (NAA). Nodal segments (1.5 cm height) were used as explants and kept in vitro MS media containing also mio-inositol (100 mg l-1) and sucrose (30 g l-1). The pH of the media was adjusted to 5.8 before autoclaving. The nodal cuttings produced callus. These callus were yellowish and friable. The highest percentage of callogenesis 69.8% was obtained with 0.07 mg l-1 NAA.

Genetic and enzymatic polymorphisms of Interspecific Dioscorea Praensilis-Dioscorea rotundata hybrides from côte d’ivoire

Yam is a crop that is found in tropical areas. It greatly contribute to populations food in these areas, namely in Africa. In Côte d’Ivoire, yam is the first among food crops with an annual production of 5.8 million tons in 2013. In spite of the importance of this food crop, it is noticed a weak level of yield due to the lack of planting material for plantation establishment. Moreover, there is a decraese of Malgré l’importance de cette culture, il est constaté à une insuffisance de la production liée au manque de semenceaux pour l’établissement de plantations.