Rp hplc method for the estimation of amikacin, aminoglycoside anti biotic

International Journal of Development Research

Volume: 
07
Article ID: 
8233
4 pages
Research Article

Rp hplc method for the estimation of amikacin, aminoglycoside anti biotic

Gurucharana Das, V.

Abstract: 

The proposed RP-HPLC method is developed and subsequently validated which is rapid, sensitive and reproducible for the analysis of amikacin in pharmaceutical dosage forms with short analysis time. A mobile phase is a mixture of acetonitrile, methyl alcohol, and o- phosphoric acid, OPA, (0.1%) in 50:35:15 (v/v).A flow rate of 1.0 mL/min mobile phase was found to be suitable in the studied range of 0.5—1.5 mL/min. The spectra of Amikacin showed a balanced wavelength at 272 nm. The linearity was found to be in the range of 1—5 μg/mL between the concentration of Amikacin and peak area response. Under the above optimized conditions a retention of 9.6 min was obtained for Amikacin. A non polar Luna C18 column (250 mm X 4.6 mm,5µ ) was chosen as the stationary phase for this study.

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