Protective effect of Lonchocarpus araripensis lectin in rat polymicrobial sepsis
International Journal of Development Research
Protective effect of Lonchocarpus araripensis lectin in rat polymicrobial sepsis
Received 30th June 2020; Received in revised form 21st July 2020; Accepted 11th August 2020; Published online 30th September 2020
Copyright © 2020, Ana Maria Sampaio Assreuy et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Objective and design: To evaluate the effect of the seed lectin from Lonchocarpus araripensis (LAL) in inflammation, clotting and vascular dysfunction in septic rats. Methods: Sepsis was induced by cecal ligation and puncture (CLP) in male Wistar rats and evaluated 24 h later: 1. Peritoneal fluid - leukocyte migration, myeloperoxidase (MPO) activity and content of malondialdehyde (MDA), NO2-, interleukins (IL-1β, IL-6, IL-10); 2. Blood - coagulation tests (aPTT and PT) and platelet aggregation (ADP); 3. Aorta - vascular reactivity to phenylephrine (Phe) and acetylcholine (ACh). Animals were treated with LAL (1 mg/kg; i.v.) 30 min before sepsis induction. Results were analyzed by Student t test or ANOVA followed by Bonferroni test (p <0.05) and expressed as Mean ± S.E.M. (n = 5-7). Results: LAL reduced total leukocytes (43%), neutrophils (39%), NO2- (100%), MDA (63%), MPO activity (70%), IL-1β (70%), IL-6 (78%) and the clotting time in the PT test (CLP: 35 ± 2.4 s vs. Sham 21.5 ± 3.5 s), and increased IL-10 (43%). LAL attenuated vascular dysfunction by the increase of ACh-induced relaxation (LAL: 0.00035 vs. CLP: 0.00016 AUC) and Phe-induced contractility (LAL: 0.0013 vs. CLP: 0.00056 AUC). Conclusions: LAL protects inflammation, bleeding and vascular dysfunction in rat polymicrobial sepsis.
