Powdered coconut water (pcw-108) as rooster semen extender

Powdered coconut water was used to preserve the integrity of the rooster's sperm cell for long periods and improve the quality of the ejaculated semen. The composition of the powder is natural, obtained from coconut water, presenting osmolarity around 500 mOsm / kg H2O and pH around 4.5 to 5.0, which were corrected for pH 7.5-8.5 and osmolarity 350-400 mOsm / kg H2O similar to seminal plasma. The filtrate is homogenized and pulverized, with high solubility. The in vitro analysis protocol of semen with the addition of powdered coconut water as a storage medium was carried out using 1:1 volumes, with fresh semen and stored for 1, 24, 48, 72 and for 96 hours at 5°C Immediately after semen collection, the sample volume was evaluated, with a mean of 316.00 ± 68.79, ranging from 150 to 450 μL, semen pH of 8.41 ± 0.28, mean osmolarity of 365, 42 ± 27.48 mOsm / kg H2O, mean fresh semen motility of 88.93 ± 5.06% and mean progressive motility of 4.46 ± 0.35. Sperm and their motility were analyzed for up to 96 hours at 5°C, showing a significant decrease from p < 0.05. The morphological structure and integrity of the sperm membrane, classified as intact (89.03%) or dead (10.97%), were examined.