Ozonized water as a dentin cleaning solution

This work aims to evaluate the antibacterial activity of ozonized water (OW) on the Streptococcus mutans and Enterococcus faecalis microorganisms and also to verify its influence on adhesive bond strength (BS), both conditions compared to 17% Trisodium EDTA; Sodium hypochlorite (HP) 2.5%; Chlorhexidine (CHX) 2% and Physiological solution (PS). The antibacterial evaluation was carried out through the Bacterial Viability Test (BVT) on the microorganisms E. faecalis and S. mutans, in the following times: T0 (soon after the bacterial inoculum placement); T10 (10min), T20 (20min), T30 (30min) and T60 (60min). For BS, 50 crowns of bovine teeth were cut and divided according to the cavity cleaning solution. Three bulk fill resin cylinders were made for each bovine crown and the test was performed after 24 hours. The failure modes were evaluated with stereoscopic loupes with a 40x magnification. The data were analyzed statistically by the ANOVA test and Tukey's post-test with a p <0.05 significance level. All the tested solutions showed antibacterial activity, except for physiological solution. For BS there were no significant differences among the groups. It can be concluded that ozonized water may be an option as a dentin cleaning solution without interfering in bond strength.