Organogenesis induction in foliar explants and nodal segments of solanum paniculatum l

International Journal of Development Research

Volume: 
11
Article ID: 
21462
4 pages
Research Article

Organogenesis induction in foliar explants and nodal segments of solanum paniculatum l

Abstract: 

Solanum paniculatum L. popularly known as jurubeba, is a shrub that has pharmacological importance. In this way, micropropagation emerges as an alternative to enable the production of seedlings on a large scale, presenting several advantages from the multiplication of genetic material, for the exchange or evaluation of germplasms, to the production of virus free seedlings. The aim of this study was to perform an efficient in vitro establishment of organogenesis from leaf explants and nodal segments of jurubeba under the effect of different concentrations of 6-benzylaminopurine (BAP) and naphthalene-acetic acid (NAA), (BAP: 0.0, 1.0, 2.0, 4.0 or 8.0 mg L-1; NAA: (0.0, 0.5 or 1.0 mg L-1). The experiment was carried out in DIC (Entirely Randomized Design), and elaborated in a 3x5 factorial scheme, showing statistically significant differences (p <0.05) for all variables analyzed. To induce greater callus formation in leaf explants and nodal segments, the use of BAP and NAA (1.0 mg L-1) is recommended. For bud formation, from leaf explants, it is recommended to promote the use of 1.0 mg L-1 NAA without using BAP. For nodal segments, a greater number of shoots is obtained with 8.0 mg L-1 of BAP without using the NAA growth regulator.

DOI: 
https://doi.org/10.37118/ijdr.21462.03.2021
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