Evaluation of antimicrobial resistance in micro-organisms isolated in surfaces of the intensive care unit of a hospital in tres lagoas-ms, Brazil

We investigated the microbiological profile and antimicrobial resistance of bacteria isolated in inanimate surfaces of the Intensive Care Unit (ICU) of a hospital in Tres Lagoas. Samples of the different surface of ten beds, equipment and instruments inside the ICU were collected. The samples (134) were seeded on blood agar, isolated and identified following a standard protocol. Were isolated 267 colonies, of which 198 were identified to the genus Staphylococus (74.15%), where Staphylococcus epidermidis was the most prevalent (62.6%), followed by Staphylococcus aureus (31.3%), Staphylococcus saprophyticcus (6 ,1%) and others coccus: Enterococcus spp (1.85%) and Micrococcus luteus (0.36%). Additionally, we identified 11 Gram negative bacilli (4.06%), where the bacteria of Enterobacteriacea family was the most prevalent mainly: Pseudomonas aeruginosa (45.46%); Acinetobacter spp. (18.18%); Enterobacter spp., Klebsiella pneumoniae, Providencia rettigeri, Serratia marcenses (9.09%). Additionally, 32 Gram positive bacilli (11.81%) were isolated, mainly: Bacillus spp. (59.37%); Bacillus cereus (18.75%); Bacillus circulans (9.38%); Bacillus coagulans (6.25%) and Bacillus subtilis (3.13%), Finally, 7 fungi (2.58%) were isolated, mainly: Penicillium spp. and Aspergillus spp. (42.86%) and Absidia spp. (14.28%). We observed moderate resistance to antibacterials in all genders analyzed and concluded that monitoring is essential for the control of pathogenic and multidrug-resistant strains.