Electrophoretic analysis of expression of trypsin inhibitors during chickpea (cicier arietinum L.) seed development

Chickpea (Cicer arietinum L.) is one of the important pulse crops in India which provides high quality proteins for vegetarians. India shares 75% of the world’s chickpea supply. Investigation on accumulation of protease inhibitors during chickpea seed development was carried out. A total of eight genotypes were investigated for expression of trypsin inhibitors during seed development by using polyacrylamide gel-X-ray film contact print method. Electrophoretic profiles of trypsin inhibitors (TI) revealed the results in which a total of 6 isoinhibitors of trypsin were detected in the mature seeds of chickpea. All the eight genotypes exhibited no expression of trypsin inhibitors at 15 days after flowering (DAF). Six genotypes (ICCL-86111, Digvijay, ICC-4958, Chafa, WR-315 and PG-9758) showed no expression of trypsin inhibitors up to 30 DAF. Two genotypes (ICCV-10 and 3137) started expressing trypsin inhibitors around 30 DAF.