Assessment of using biologic surgical adhesive (bioglue) on preventing urine leakage after surgical repair of hypospadias in animal dog model

International Journal of Development Research

Volume: 
08
Article ID: 
12815
8 pages
Research Article

Assessment of using biologic surgical adhesive (bioglue) on preventing urine leakage after surgical repair of hypospadias in animal dog model

Zeraatian, S., Mesbah, M., Hosseini, K., Hosseini, M., Kamalzadeh, N. Naseripour, M. and Pazooki, D.

Abstract: 

Aim in this study was to evaluate the protective effect of Bioglue for suture line in the repair of hypospadias. Back ground: Bioglue has been used in the treatment of many surgical diseases. The main purpose of Bioglue was as protective layer for traditional suture line or replacement of traditional suture line. Different studies evaluated different methods and techniques to reduce urethra-cutaneous fistula which is a major complication of hypospadias repair. But review of literatures reveals that they were not successful completely. Materials and Methods: 16 dogs (4 to 6kg German Shepherd) enrolled in this study in animal lab of Shiraz University of Medical Sciences. Dogs were divided into two groups. Group I repaired with a layer of Bioglue between the protecting flaps and Group II without it; repair performed in fine manner with PDS 6-0 in both groups. One case and one control dog were sacrificed after two weeks then the remaining sacrificed in two weeks intervals sequentially. Urethra was checked grossly and radiological for leakage. Then penis harvested and underwent histopathologic evaluation with light microscope by Hematoxylin and Eosin staining. Results: Two out of eight (25 %) urine leakage confirmed radiological and grossly in GII versus no leak in GI (Predictive Value of 0.23). There were No evidence of ossification, stenosis, stricture or increased collagen formation in both groups (II, I) and all tissue healing process were same. Conclusion: We recommend using Bioglue in repair of hypospadias in animal in further larger studies to re-evaluate its effect on protection of suture line. If future studies confirm its role in animals, we can use it not only as a sealant layer but also as scaffold that helps better wound healing for prevention of fistula formation in human hypospadias repair.

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