Metabolic turnover of carbohydrates during pupal-adult transition stage in the silk worm, bombyx mori

The present study has been focused on determination of various constituents of carbohydrate’s metabolism during pupal-adult transition stage in the silk worm, Bombyx mori. Vth instar larvae of the silkworm, Bombyx mori (DFLs), obtained from the local grainages were maintained under laboratory conditions of 260C-280 C temperature range according to the guidelines of Krishnaswamy (1978).The pupae and adult male & female moths were sacrificed on selected days viz.1st, 3rd, 5th, 7thand 9thdays and two selected tissues such as fat body and haemolymph were collectedand stored at -700C. At the time of biochemical analysis, the tissues were thawed and used. Various constituents of the Carbohydrate metabolism viz. Total carbohydrates, glycogen and Trehalosealong with their associated enzymes such as Alpha-amylase and Trehalase activity were estimated in the Fat body and Haemolymph as per the standard methods. Biochemical variations, observed in carbohydrates during the 9 day duration of pupal life and also during pupal-adult transition period indicated utilization of carbohydrates for energy production. Present finding clearly established the obsorption and also exchange relationship of biochemical constituents between fat body and haemolymph, of course a significant differences in male and female moths. These Sex-specific variations in biochemical constituents of carbohydrate metabolism correlated well with the energy demands of male and female adults.