Antimicrobial resistance detection of citrobacterfreundii isolated from rectal swab in east java
International Journal of Development Research
Antimicrobial resistance detection of citrobacterfreundii isolated from rectal swab in east java
Received 05th August 2017; Received in revised form 29th September, 2017; Accepted 18th October, 2017; Published online 30th November, 2017
Copyright ©2017, Intan Permatasari Hermawan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The aim of this study was to detect blaTEMgene which the agent of antibiotic resistance in Citrobacterfreundii isolated from rectal swab in East Java. A total of 275 rectal swab samples were collected from 12 district of East Java. Then, samples were isolated with selective media and biochemical tests. Antibiotic Resistance test was using the disc diffusion against amoxycillin, amoxycillin-clavulanic acid, ampicillin-sulbactam, cefotaxime, ceftazidime, sulfametaxole-trimethoprim, and tetracycline and then detection of bla TEM gene used PCR. The number of Citrobacterfreundii was 7 (2,55 %). Antimicrobial resistance showed 2 broilers and 1 dairy cattle from Surabaya resistant to ampicillin-sulbactam and tetracycline, 4 milkfish from Sidoarjo resistant to amoxicillin and ampicillin-sulbactam. All seven samples were positive blaTEM gene with 445 bp size used PCR.
