Antimicrobial effect of pyomelanin extracted from pseudomonas aeruginosa
International Journal of Development Research
Antimicrobial effect of pyomelanin extracted from pseudomonas aeruginosa
Received 25th January, 2017; Received in revised form 09th February, 2017; Accepted 14th March, 2017; Published online 30th April, 2017
Copyright©2017, Zainab Radhi Abdul-Hussien and Sanaa Saeed Atia. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Pigmented bacterial isolates was isolated from various environmental samples. Vitek identification analysis and PCR analysis of 16SrRNA amplifying gene showed that this bacteria was related to Pseudomonas aeruginosa. Pigments was extracted with 1N HCl and ethanol.After extraction, pigment was subjected to various chemical analysis including thin layer chromatography, spectrophotometric analysis and gas chromatography –Mass spectrophotometric analysis. Chemical analysis showed that pigment was characterized as, pyomelanin which was evaluated biologically to examine its cytotoxicity against red blood cells, results showed that pyomelanin had no cytotoxic effect. Also pyomelanin was tested against pathogenic bacteria and fungi. Statistical analysisof present results showed thatpyomelanin gave an antibacterial and antifungal activity.
