Methods for studying microbial biofilm

Microbial biofilms are multicellular community formed by microorganisms as bacteria and/or fungi involved by polymeric substance called extracellular matrix. The extracellular matrix protects microbes against environments external threats and it facilitate the interaction between others pathogenic and symbiotic organisms. Thus, these communities of microorganismsare medical and environment importance that stimulates the study of biofilm in last years. However, the biofilm life-style is still poorly understood, and more researches are necessaries to elucidate the development of microbial biofilms. Therefore, the aim of this paper is to concatenate the methods of evaluating the development of microbial biofilm in vitro through Staining assays, techniques of Morphology and Molecular Biology.