Macroscopic effect of lipoic acid in skin injury in the model of diabetes mellitus induced by alloxan in rats

International Journal of Development Research

Volume: 
10
Article ID: 
18436
7 pages
Research Article

Macroscopic effect of lipoic acid in skin injury in the model of diabetes mellitus induced by alloxan in rats

Luis Rafael Leite Sampaio, Emanuel Messias Silva Feitosa, Vithória Régia Teixeira Rodrigues, Valeska Edith LucasLeal, Lucas Teixeira Nunes Borges, Talita Matias Barbosa Cavalcante, Gyllyandeson de Araújo Delmondes, Francisca Clarisse de Sousa, Silvânia Maria Mendes Vasconcelos, Marta Regina Kerntopf, Rita Neuma Dantas Cavalcante de Abreu, Karla Maria Carneiro Rolim and Cláudio Gleidiston Lima da Silva

Abstract: 

Objective: Evaluate the macroscopic effect of lipoic acid on skin lesion in rats with alloxan-induced diabetes mellitus model. Material and Methods: For diabetes induction, Wistar albino rats received Alloxan at 50mg/kg. After 72 hours, the glycemia was verified to confirm diabetes; animals with values below 250mg/dl were discarded. After the diagnosis, surgical excision of a skin fragment was performed with a 7 mm punch. Following the procedure, the animals were divided into three groups containing five animals each, which received: oral distilled water and cellulose (control) or Alpha-Lipoic Acid (ALA) (100mg/kg or 200mg/kg) for 1, 7 or 14 days of treatment. At the end of each treatment period, a evaluation of the following parameters was performed: infection; hyperemic halo around the wound; crust formation; necrotic border, bleeding background and measurement of contraction percentage. Results: The ALA treatment reduced, in both doses, the crusts formation in the stipulated periods for evaluation. While ALA 100mg/kg increased the contraction percentage of the lesion at all times studied. A similar results was obtained with ALA 200mg/kg after 7 or 14 days of treatment. Conclusion: ALA presented satisfactory results in the healing process of wounds in diabetic rats.

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