Comparison of three diagnosis techniques of hirschsprung disease

Hirschsprung disease (HD) is a congenital disease caused by the absence of ganglion cells in the large intestine leading to functional obstruction and colonic dilatation. This work focuses on showing the scientific evidence on the evaluation of two diagnostic techniques of rectal biopsy and compares the advantages and disadvantages of each. Bibliographic research was performed using Scielo, Medline, and academic Google, related to Hirschsprung disease, Immunohistochemistry Calretinin (IHC), and Histochemistry Acetylcholinesterase. The articles were chosen for their relevance on the topic, for their advantages and disadvantages mentioned between the two techniques that have been mentioned, and related to the variants that manifest the pathology. We observed that in normal people, acetylcholinesterase (AChE) activity is minimal or nil, butin patients with HD, an increase in enzyme activity was observed in the layers of the large intestine.Furthermore, histochemical staining can produce a dark brown positioning in fibers with AChE activity on histopathological analysis (positive pattern of HD). In turn, immunohistochemistry uses calretinin. In the ganglionic parts of the intestine, there is an expression of calretinin in the submucosa, and in the aganglionic parts, there is none. Therefore, we conclude that the IHC has more advantages and greater or equivalent diagnostic accuracy than the Histochemical technique with AChE. In turn, immunohistochemistry uses calretinin. In the ganglionic parts of the intestine, there is calretinin expression in the submucosa, and in the aganglionic parts, there is none. Therefore, we conclude that the IHC has more advantages and greater or equivalent diagnostic accuracy than the Histochemical technique with AChE. In turn, immunohistochemistry uses calretinin. In the ganglionic parts of the intestine,calretinin is expressed in the submucosa, and in the aganglionic parts, there is none. Therefore, we conclude that the IHC has more advantages and greater or equivalent diagnostic accuracy than the Histochemical technique with AChE.