Is acid citrate dextrose solution (acd) better than others anticoagulants for pseudothrombocytopenia?

Pseudothrombocytopenia is a worrying clinical phenomenon that can result in an error of diagnosis and inadequate therapeutic conduct, if the necessary knowledge to differentiate this condition from the real condition does not act. It is not known for certain what causes this condition. The mean platelet volume (MPV) and the normal bleeding time indicate that the platelet decrease is induced during the in vitro process. It is worth mentioning that the phenomenon of thrombocytopenia linked to the EDTA anticoagulant is not permanent, and may fluctuate periods when false thrombocytopenia is detected or not. This is due to the unknown pathophysiology of antibody production. Some researchers attribute the phenomenon of platelet IN VITRO agglutination only when the EDTA anticoagulant is present. Because of this, a clinical laboratory protocol will be developed when Pseudothrombocytopenia is suspected. The simple method consists of shaking the EDTA tube in the vortex for approximately one minute in order to dissolve the platelet aggregates, and to be reprocessed in the equipment if it is not efficient, a recollection using other tubes with alternative anticoagulants such as, for example, sodium citrate or ACD. These two tubes have shown extremely positive results in preserving the coagulation sample. The aim of this study is to evaluate the effectiveness of the ACD anticoagulant in relation to EDTA and sodium citrate anticoagulants in patients who present with pseudothrombocytopenia through a data survey. As a methodology, medical records will be used anonymously for patients from the Clinical Analysis Laboratory - Campinas / SP, who are laboratory-dependent EDTA. The medical records will be evaluated retroactively for a period of six months to assess laboratory data against the different anticoagulant effects. The data will be compiled and analyzed with statistical software and shows that ACD is better than the others anticoagulants.