In vivo and ex-vivo spatiotemporal expression of stem cell markers in mouse embryonic brain cells

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Author: 
Cristiane Valverde Wenceslau, Diana Aparecida Câmara, Damiana Pedro de Oliveira, Celine Pompeia, Gustavo Sabino Lisboa and Irina Kerkis
Abstract: 

We investigated the expression of pluripotent stem cell (PSC) and neural stem cell(NSC) markers in mouse embryonic brain cells at embryonic (E) days9.5, E12.5, E15.5 and E18.5, after NSC isolation and growth in vitro and in the adult brain. Biomarkers such as Oct-4, Sox2, Sox1, Fragilis and nestin are expressed in vivoat different stages of embryonic brain development and are also found in the adult brain. Oct-4 co-localization to chromosomes was found at E9.5 in symmetrically-dividing neuroepithelial cells and at E12.5, in primary brain vesicles. Only few cell express Nanog at E9.5 and E18.5. In vitro cultured pNSCsare Oct-4+, Sox+, Nanog+, Fragilis+, Pax6+ and nestin+. However, the expression of Oct-4, Sox2 and Nanog is very low in pNSCs as compared to that of embryonic stem cells (ESCs). These cells are committed to the neural fate,can form neurospheres and differentiate spontaneously into neuronal cells in vitro. In contrast to PSCs, which also express the aforementioned markers, pNSCs are unable to form teratomas in nude mice. The use of these PSCs biomarkers may help trace NSC course and fatein brain development, as well as promote further identification and in vitroisolation of new lineages of PSCs and NSCs.

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