UV/VIS Spectrometry with multivariate calibration as a new approach for the Chiral analysis of Fluoxetine

Nowadays, a large number of chiral analyses are needed, simple and rapid methods for the determination of the enantiomeric composition in pharmaceutical products should be developed. The fluoxetine belongs to the most prescribed antidepressant chiral drugs and its enantiomers have a different duration of serotonin inhibition. This study presents cheap and fast alternative to traditional chiral techniques for the determination of the fluoxetine enantiomeric composition by the combination of UV/VIS spectrometry and multivariate calibration. The chiral recognition of the fluoxetine was based on the creating of the diastereomeric complexes with α- and β- cyclodextrin. Multivariate calibration methods, including principal component regression (PCR) and partial least square method (PLS), were used for spectral data evaluation. Small differences in results between eachcyclodextrin and both calibration models were obtained by multivariate calibration. PLS model for determination of the enantiomeric composition of fluoxetine by β-cyclodextrin as a chiral selector had slightly better prediction than others models. Predicted values of the enantiomeric composition in the synthetic samples are similar to their nominal values.