Stability indicating rp-hplc determination of curcumin in vicco turmeric cream and kasturi turmeric churna

A simple, specific, precise and stability-indicating RP-HPLC method was developed and validated for analysis of Curcumin in bulk drug, cream and churna formulations. Chromatographic separation were achieved using Agilent-TC C18 column (250 X 4.6 mm; 5µ) column at ambient temperature. Mixture of methanol, acetonitrile, and 5% acetic acid (35: 50: 15, v/v) was used as mobile phase at constant flow rate of 1.0 ml/min and 420 nm was selected as wave length for detection of method. The Curcumin peak was obtained at RT 4.92 min. The linear regression analysis data for the calibration plots showed good linear relationship with r = 0.9993, in the concentration range 80–120 µg/ml. The method was validated for specificity, precision and recovery. LOD was found to be 1.67 µg/ml and LOQ was found to be 10.28 µg/ml. Curcumin was subjected to acid, neutral and alkali hydrolysis, oxidation, thermal, UV light and humidity degradation and indicates that the drug is not susceptible. As the developed method effectively separated the drug peak from its degradation products, shows that method is specific and stable. The developed method can be used in pharmaceutical industry for routine analysis of Curcumin in cream and churna based formulations.