Rp hplc method for the estimation of amikacin, aminoglycoside anti biotic
International Journal of Development Research
Rp hplc method for the estimation of amikacin, aminoglycoside anti biotic
Received 24th December, 2016; Received in revised form 04th January, 2017; Accepted 21st February, 2017; Published online 31st March, 2017
Copyright©2017, Gurucharana Das. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The proposed RP-HPLC method is developed and subsequently validated which is rapid, sensitive and reproducible for the analysis of amikacin in pharmaceutical dosage forms with short analysis time. A mobile phase is a mixture of acetonitrile, methyl alcohol, and o- phosphoric acid, OPA, (0.1%) in 50:35:15 (v/v).A flow rate of 1.0 mL/min mobile phase was found to be suitable in the studied range of 0.5—1.5 mL/min. The spectra of Amikacin showed a balanced wavelength at 272 nm. The linearity was found to be in the range of 1—5 μg/mL between the concentration of Amikacin and peak area response. Under the above optimized conditions a retention of 9.6 min was obtained for Amikacin. A non polar Luna C18 column (250 mm X 4.6 mm,5µ ) was chosen as the stationary phase for this study.
