Retrieval of plantlets from cryopresesrved alginate buds of Nyctanthes arbor-tristis L.- an effectual approach for germplasm conservation

The escalating exploitation of medicinal plants for the extraction of imperative pharmaceuticals amalgam dictated the need of using encapsulated vegetative propagules for germplasm conservation and propagation. The present report is the first attempt in the production of synseed and subsequently affirming retrieval of healthy plantlets of Nyctanthes arbor-trisits L. In the current exploration, best encapsulated nodal segments were developed in calcium alginate gel composed of 3% (w/v) sodium alginate and 100 mM CaCl2.2H2O. Maximum conversion response (76.66%) with maximum number of shoots (4.33 ± 0.57) was obtained on MS medium augmented with KN (2.5 µM) + NAA (0.5 µM). These encapsulated axillary buds ensured greatest retrieval (86.66%) of shoots after a period of 4 weeks spend in cold storage storage at  4 ᵒC. Microshoots were then excised and rooted in half-strength MS medium (pretreated with 200 µM IBA). Well-developed plantlets were acclimatized successfully with 90% survival frequency.