Production of thermostable fungal amylase and its application in the discoloration of indigo carmine

The indigo carmine dye is widely used within the textile industry, however its high toxicity for bringing several harms to humans and the environment. To minimize the consequences of its use, several methods have been studied, among them the degradation by biological agents has been of great interest. Amylases, which are hydrolytic enzymes commonly used in the textile industry, can be quite effective in the biodegradation of dyes. This study aims to produce amylases with broad stability and ability to discolor of dyes used in the industry. In the present study to optimize the production of amilase complex it was performed a central experimental design compound (23) with three central points and variables pH values, and concentration of starch. After it was characterized according to the effect of pH and temperature on the reaction and amylolytic stability. Thus, it was produced at 30ºC using starch 0.25% as an additive to the culture medium, a thermostable amylase, which hydrolyzes starch in a wide pH range (3.5-7.0) and high temperatures (50º and 60ºC). When analyzing the biodegradation of the indigo carmine dye, it showed high efficiency, decolorizing it about 92% in 48 hours.