Phytochemical screening and antioxidant activity of leaves and cell suspension culture from seed derived callus of Spinacia Oleracae

Kamini S. Yeole, Anirudh P. Bhagat, Anita V. Handore, Sharad R. Khandelwal and Vinita S. Jagtap

Objective: To screen the phytochemical constituents as well as to assess the total phenolic content and antioxidant potential of plant extract(leaves) and cell suspension culture extract from the seed derived callus of Spinacia oleracae.
Methods: Establishment of callus was carried out by use of MS medium supplemented with growth hormones, BAP and IAA with some modifications in the standardized method. Plant extract was subjected to the phytochemical screening as reported methods. Folin–Ciocalteu reagent was used to estimate total phenolic content and the antioxidant potential was determined by DPPH radical scavenging assay.
Results: Phytochemical screening of extract showed presence of major classes of phytochemicals such as Alkaloids phenols, tannins, flavonoids, terpenoid, carbohydrates, glycosides, reducing sugars and proteins. It was found that the seed derived callus formation was maximum after 28th day and satisfactory establishment of cell suspension culture was observed after the 25th day of inoculation. An antioxidant potential of cell culture extract was found to be 75.78 %where the value was 68.47% for leaf extract.Total phenolic content of plant extract (leaves) and cell suspension culture extract was found to be 4.85mg GAE/g and 3.96 mg GAE/g respectively.
Conclusion: The result revealed that the leaf extract showed higher total phenolic conent as compare to cell culture extract However,an antioxidant potential of leaf extract was found to be lower than the cell culture extract. The possible reason for increase in anioxidant potential of cell culture extract may be due to stimulation of biosynthetic pathways of primary and secondary metabolites which may be responsible for antioxidant activity. Thus the present study will be useful for application of cell culture derived from spinach seeds , as a promising alternative to the whole plant for the large scale production of plant phenolics.

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