A paternity case based on short tandem repeat (str) using dna finger printing technology

DNA Fingerprinting is the technology which is used to identify each individual on the basis of the molecular characteristics of their DNA. The technologies and applications have continued since 1985. Every individual in the world can be identified at the molecular level on the basis of an extremely greater level of polymorphism in the sequence of DNA which is inherited from biological parents and is identical in every cell of the body. DNA fingerprinting, as this technique of identification is called, can confirm with certainty the parentage of an individual. The method of DNA profiling is carried out currently by the polymerase chain reaction (PCR) based short tandem repeat (STR) profiling. STR technology is used to evaluate specific regions (loci) within the DNA. Variability in STR loci can be used to distinguish one DNA profile from another. The amplification of these microsatellite polymorphisms STR loci has progressed from single locus reactions via multiplex PCR reactions (CTT triplex and Silver triplex), amplifying as many as sixteen loci in one reaction tub. In parallel the detection of STR loci has advanced from polyacrylamide gel electrophoresis (PAGE) and silver stain techniques. This test was carried out by obtaining the dried blood spots from the mother, baby and alleged father. The child and the alleged father were tested. The paternity case analysis was performed on six STR (THO1, TPOX, CSF1PO, D16S539, D7S820 and D13S317) loci. In the DNA profiles tested, when the mother's alleles were excluded in the child's STR profile, the remaining alleles matched with the putative father's profile, thus confirming the paternity of the putative father.