Optimization of culture conditions for tannase production by Streptomyces Sp.

Tannin Acyl Hydrolase (E.C 3.1.1.20) commonly referred as tannase is an industrially important enzyme which is mainly used in the food, chemical, pharmaceuticals and beverage industry; it is produced by number of microbes, animals, and plants. In this study production of tannase was investigated using agro-residue, Azadirachta indica leaves as a substrate of hydrolysable tannin by potent tannase producer isolate Streptomyces sp.SKA1. Optimization of culture conditions for maximum tannase production included studying the effect of age of inoculum, inoculum size, incubation period, incubation temperature, pH, concentration of agro-residues, concentrations of carbon and nitrogen sources on enzyme activity. The optimum culture conditions determined were Inoculum age-5 days (120 hrs), inoculum volume -6 %, incubation period-5 days (120 hrs),  incubation temperature - 30°C, pH - 7, agro-residue concentration – 4%, as a substrate,  starch- 1% (w/v)  as a carbon source and casein- 0.03% (w/v)  as a  nitrogen source for maximum tannase activity.