Defensin Gene Expression Levels (CBD1 and CBD103) in skin scrapings of the poodle dogs with and without dermatitis
International Journal of Development Research
Defensin Gene Expression Levels (CBD1 and CBD103) in skin scrapings of the poodle dogs with and without dermatitis
Received 18th June 2020; Received in revised form 19th July 2020; Accepted 27th August 2020; Published online 23th September 2020
Copyright © 2020, Alessandra C. O. Pinheiro et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Defensins represent an important group of antimicrobial peptides and are expressed in the skin and other tissues of various mammalian species. These peptides participate in innate immunity, aiding in the immune response. The objective of this work was to compare the levels of β-defensin gene expressions (cBD1 and cBD103) in skin scrapings of the Poodle dogs with and without dermatitis. Eight dogs with dermatitis and 13 healthy dogs from the Veterinary Hospital and privately owned had collected skin scrapings. RNA extraction was obtained by Trizol® reagent. mRNA expression levels were estimated by RT-PCR using the SYBR® Green One-step Kit on the CFX96 Touch ™ Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA). Data were compared by 5% Student's t-test. Relative cBD1 and cBD103 genes expressions were significantly higher in dogs with dermatitis (P<0.05). Dogs with dermatitis showed higher levels of defensin expression, suggesting a possible immune response in the skin of these animals.
