Isolation and characterization of amylase producers and optimization of enzyme production
International Journal of Development Research
Isolation and characterization of amylase producers and optimization of enzyme production
Received 18th September, 2017; Received in revised form 27th October, 2017; Accepted 20th November, 2017; Published online 30th December, 2017
Copyright ©2017 Murali Krishnan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The soil sample was screened for isolating amylase producing soil bacteria. The isolates were observed for microscopic and selected biochemical tests for characterization. The results of gram staining reveal that the strains VO1, VO2 and VO3 were gram positive rods. The initial amylase production was confirmed by inoculating the isolates in Berg’s broth. The organisms were acclimatized to higher temperatures to evolve thermal stable enzyme by inoculating g the colonies at 400, 450 and 500C.
