Antioxidant activity of nutmeg bark (myristica fragrans houtt)
International Journal of Development Research
Antioxidant activity of nutmeg bark (myristica fragrans houtt)
Received 18th June, 2017; Received in revised form 19th July, 2017; Accepted 24th August, 2017; Published online 30th September, 2017
Copyright ©2017, Mustanir et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
This study would be focused on nutmeg bark (Myristica fragrans Houtt) isolation to observe antioxidant activity of nutmeg bark (NB). The result of phytochemicals screening test showed that metabolite secondary compound of NB were terpenoid and tanin. Antioxidant activity from the n-hexane extract of nutmeg bark (n-HENB) was conducted using the DPPH method. Antioxidant activity of n-HENB with DPPH at concentration of 25, 50 and 100 ppm is obtained IC50 63.76 ppm. The results of column chromatography of n-HENB obtained 11 combined fraction, that were fraction of A to K. The results of antioxidant activity of IC50 with a very strong category was the fraction of H and K with 52.04 and 42.21 ppm, respectively. It showed that the fraction of the NB had a better inhibition due to the presence of some active compounds contained in fractions that can synergize in inhibiting free radicals, so that the IC50 value of the fraction is more powerful than the extract.
