Use of homeopathic eye drops containing euphrasia officinalis to treat eye inflammations – In Vitro proof of concept

Integrative medicine has increasingly embraced homeopathy due to its reported effectiveness against various diseases without the associated side effects commonly seen with allopathic medications. Euphrasia officinalis has been recognized for over 70 years for its beneficial properties in addressing eye disorders. This study's objective is to modulate the inflammatory process using Euphrasia 6CH eye drops in an in vitro setting. The medication was administered to the human mesenchymal stem cell culture after the induced cellular inflammation by LPS. The concentration of the product where no cytotoxic effects were observed (8 µL/mL) was assessed. Then, after culturing the cells, inflammation was induced using LPS, followed by treatment with the medication. The supernatant was collected, and the levels of cytokines associated with the inflammatory process were measured using the flow cytometry method. After the treatment, cytokines quantification revealed a statistically significant decrease in proinflammatory cytokine levels (IL-6 e IL-8). These results indicate that Euphrasia 6CH eye drops decreased proinflammatory cytokines. Consequently, further tests could explore diverse applications of this medication beyond its use in conditions like conjunctivitis.