New access of pulmonary fibrosis induced by bleomycin in rats

Background: Pulmonary fibrosis (PF) is a disabling and life-threatening disease, involved in inflammatory processes which proceed in fibroblast proliferation and consequent increase of connective tissue in lung parenchyma, leading to deterioration of function. Objective: to determinate the effectiveness of a new method of inducing pulmonary fibrosis with bleomycin (BLM) injected into the lumen of the trachea aided by palpation in rat. Materials and methods: The sample was consisted of 18 rats subjected to induced pulmonary fibrosis and their controls. After 3 days the animals were euthanized and lungs removed, which were processed for morphological analysis of the inflammatory infiltrate and check total collagen content of the lung hydroxyproline count in tissue. The GraphPad Prism 1.6 software was used for statistical analysis with ANOVA ONE WAY test with Tukey post-test, p values <0.05 were considered significant. Results: It was found that the inflammatory process induced by BLM behaved with infiltration of inflammatory cells reflecting inflammation and increased hydroxyproline with a significant difference. Conclusion: The new method proved to be a simple and efficient method for PF induction.