Molecular profile of oral candida albicans isolates from hiv-infected patients and healthy persons
International Journal of Development Research
Molecular profile of oral candida albicans isolates from hiv-infected patients and healthy persons
Received 19th May, 2017; Received in revised form 27th June, 2017; Accepted 10th July, 2017; Published online 30th August, 2017
Copyright ©2017, Anusha Reddy and Ajit Reddy. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Candida species are part of the normal microbiota of most people, but can also be responsible for infections, acting mainly as opportunistic microorganisms. The typing of Candida isolates is important for hospital epidemiology studies. The aim of this study was to analyze the molecular profile of Candida albicans isolates from the oral cavity of individuals with and without HIV and verify genetic relationships between them using the RAPD-PCR methodology. Two different groups, containing a total of 116 C. albicans isolates, were analyzed. Group 1 contained 75 isolates from saliva samples from HIV patients with no clinical signs of oral candidiasis, and Group 2 contained 41 isolates from healthy volunteers without HIV. Every isolate was typed by RAPD-PCR using the random primers OPA9 and OPB11. The genotypic analysis showed 33 different genotypes defined by OPA9 and 34 by OPB11. The primer OPA9 defined 26 genotypes with the isolates from Group 1, and 21 genotypes from Group 2. This study reveals that the genetic diversity of C. albicans isolates from HIV-positive individuals may be more complex than in seronegative individuals.
