Molecular identification of dengue virus serotypes 1 and 3 in Amazonas State, Brazil during 2001–2002
International Journal of Development Research
Molecular identification of dengue virus serotypes 1 and 3 in Amazonas State, Brazil during 2001–2002
Received 10th August, 2022; Received in revised form 22nd August, 2022; Accepted 24th September, 2022; Published online 30th September, 2022
Copyright © 2022, Luís Felipe Alho da Silva et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Objective: Investigate DENV circulation in the serum of patients with febrile illness presented at the Fundação de Medicina Tropical Doutor Heitor Vieira Dourado (FMT-HVD), Manaus -Amazonas between 2001–2002. Methods: 116 sera collected in the acute phase were tested by semi-nested multiplex PCR. Results: Among the 116 patients, 46 were positive for DENV: 6 DENV1 and 40 DENV3. Moreover, among the DENV3 samples collected in 2001, only two patients reported travel to communities near Manaus. Conclusions: This study reported the molecular detection of DENV1 and DENV3 and the circulation of DENV3 in autochthonous cases since 2001. These findings reinforce the importance of laboratory approaches in the surveillance of emerging and re-emerging diseases.
