Isolation of protein from cell free culture filtrate: A noble approach

×

Error message

  • User warning: The following theme is missing from the file system: journalijdr. For information about how to fix this, see the documentation page. in _drupal_trigger_error_with_delayed_logging() (line 1138 of /home2/journalijdr/public_html/includes/bootstrap.inc).
  • Deprecated function: implode(): Passing glue string after array is deprecated. Swap the parameters in drupal_get_feeds() (line 394 of /home2/journalijdr/public_html/includes/common.inc).
  • Deprecated function: The each() function is deprecated. This message will be suppressed on further calls in _menu_load_objects() (line 579 of /home2/journalijdr/public_html/includes/menu.inc).

International Journal of Development Research

Isolation of protein from cell free culture filtrate: A noble approach

Abstract: 

Bacteria are well known for their ability to liberate proteins and enzymes into their environment. Pseudomonad sp. being important micro-organisms produces wide range of extra-cellular enzymes including proteases. The pseudomonad strains R62 and R81 has been used in this study for electrophoresis analysis of extracellular protease enzymes which were present in the cell free culture of nutrient broth (NB) and protease specific broth (PB) media. The SDS-PAGE analysis of the crude protein from cell free culture filtrate from both the media when analyzed for proteases, showed the presence of different bands in the electrophoretic field.

Download PDF: 
PDF icon 3043.pdf