Isolation, Characterisation and biofilm production of e.coli from mackerel (rastrelliger Kanagurta)

Author: 
Leonal Rabins, S., Bhattacharya, A. Ajay Kumar, V.J. and Vijayan, C.
Abstract: 

Fish and fishery products are of great importance for human nutrition worldwide and provide clear health benefits. Fish can act as a source of food borne pathogens and may be a potential source of infection. A study was undertaken to find out the incidence and characterization of E.coli in fishes, determine biofilm production and antibiotic resistance of isolates. A total of 100.0 raw mackerel fish (Rastrelliger kanagurta) was collected from different markets in and around Puducherry. Fifty two (52.0%) isolates of E.coli were recovered from 100.0 samples. Among the 52.0 isolates 38.0 (73.07%) were found to produce biofilm on modified congo red agar. All the 52.0 isolates were subjected for antibiotic resistant profile test. Of the 52.0 E. coli isolates characterized, all (100.0%) of the isolates displayed sensitivity towards norfloxacin (10 μg), sulphametoxazole/trimethroprim (25 μg) and chloramphenicol (30 μg). On the other hand, resistance was observed against ampicilin (34.3%), tetracycline (22.5%) and Amphotericin B (10.8%). The result indicates that there is an increased prevalence of E.coli mainly due to contamination of the fishes during processing and also from an environment.

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   Vol. 07, Issue 02, February 2017

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