Isolation and identification of Aeromonas spp. from drinking water in basrah governorate

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International Journal of Development Research

Isolation and identification of Aeromonas spp. from drinking water in basrah governorate

Abstract: 

Two hundred and twenty two water samples were collected from August 2008 to April 2009, which including 49 samples from 13 water purification plants in Basrah governorate (13 samples prior entering to the plants, 13 from precipitation, 10 from filtration tanks and 13 samples from the water coming out of these plants). Furthermore 127 samples of tap water were collected from 18 districts around Basrah governorate. Also 46 samples were collected from tankers which supplying Reverse Osmosis (RO) in 19 different places and from five RO water supplying plants in the governorate. The concentration of residual chlorine was measured at the time of collection with a range of 0- 1.32 mg/L. The Membrane Filtration method was used to filtered 5ml of each sample of plants and tap water and 100 ml of water samples of RO plants and tankers. The filter papers were placed on both M-FC agar for fecal coli form and Ampicillin Dextrin Agar with Vancomycin (ADA-V) medium for Aeromonas and then incubated at different temperature according to the requirements of these bacteria. 208 isolates on ADA-V medium have been identified as Aeromonas spp. by morphological and biochemical characters. Six species of Aeromonas spp. were diagnosedas: A. hydrophila (64( isolates, A. caviae )53(, A. schubertii )34(, A. eucrenophila (25), A. encheleia (17) and A. veronii bv. veronii (15). Antibiotic susceptibility tests were done for the types of Aeromonas bacteria isolated from water samples. All Aeromonas spp displayed multiple drug resistance, particularly to Ampicillin, Vancomycin and Cefixime. The presence of such pathogens in drinking water is a strong indicator of unsuitability for drinking and requires appropriate solution.

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