Isolation and characterization of bacterio phagesagainst multiple drug resistant pseudomonas aeruginosa with using the bacteriophage as a therpy in the mice model

International Journal of Development Research

Volume: 
07
Article ID: 
11519
9 pages
Research Article

Isolation and characterization of bacterio phagesagainst multiple drug resistant pseudomonas aeruginosa with using the bacteriophage as a therpy in the mice model

Mays B. Jalil, Hayder A. Al-Hmudi, Labeed Abdallah Al-Alsaad and Zeinab R. Abdul-Hussein

Abstract: 

One of the most common pathogens that are multidrug resistant and colonize burns wounds is Pseudomonas aeruginosa. This led researchers to find alternative treatment, so bacteriophages were alternative treatment methods used against bacterial infections. The phages were isolated from sewage and urine. Morphology of purified phages was examined by using the transmission electron microscopy. The titer of phage was determined by using plaque assay by serial dilutions (10-1 to 10-12).The therapeutic effectiveness of phage for treatment of mice infected with MDR P. aeruginosa was determined. Four types of phages were isolated from sewage and urine. The our results showed that giving of the dose phage injection and orally was very effective after the bacterial challenge, this has helped in the speed of recovery, so that reduced the death rates. Blood culture of mice treated with phage dose appeared no viable P. aeruginosa cells in their blood compared to the positive groups. Our study present evidence in murine models that animals infected with MDRs P. aeruginosa can be successfully treated with specific bacteriophages that target these MDRs microbe.

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