Interplay between hyperosmotic stress and protein kinase a in regulation of ciliary beat frequency in vitro

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International Journal of Development Research

Interplay between hyperosmotic stress and protein kinase a in regulation of ciliary beat frequency in vitro

Abstract: 

Ciliary beat frequency is an important component of mucociliary clearance. Our aim was to test the hypothesis that hyperosmotic stress reduces ciliary beat frequency and that this reduction can be influenced by protein kinase A manipulation in vitro. Using ciliated nasal epithelial cells ciliary beat frequency was measured at 32oC under different osmotic conditions with or without protein kinase A manipulation. In isoosmotic media at 32oC protein kinase A activation stabilised ciliary beat frequency near baseline and its inhibition significantly reduced ciliary beat frequency. Reversible reduction in ciliary beat frequency on exposure of cells to a hyperosmotic solution of 400mM mannitol at 32oC occurred; and when protein kinase A was activated or inhibited in this hyperosmotic solution ciliary beat frequency was further lowered. In conclusion under isosmotic conditions protein kinase A activation had a positive effect on ciliary beat frequency and its inhibition reduced ciliary beat frequency. Under hyperosmotic stress ciliary beat frequency was reduced and protein kinase A activation or inhibition had a negative impact in that the frequencies were lowered further though this was more pronounced when there was activation.

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