Improved developmental potential of porcine Blastocyst by l-Carnitine supplementation

×

Error message

User warning: The following theme is missing from the file system: journalijdr. For information about how to fix this, see the documentation page. in _drupal_trigger_error_with_delayed_logging() (line 1138 of /home2/journalijdr/public_html/includes/bootstrap.inc).

International Journal of Development Research

Improved developmental potential of porcine Blastocyst by l-Carnitine supplementation

Abstract: 

Objective: In this study, the potential role of L-carnitine supplementation in the maturation and preimplantation development was investigated using porcine as a model.
Materials and Methods: In Expt. 1, porcine oocytes were matured in the presence of L-carnitine (1.0, 0.5. 0.25 mg/ml) or absence (control), subjected to in vitro fertilization and assessed on their developmental potential up to the blastocyst stage. In Expt. 2, zygotes derived from in vitro fertilization were cultured in NCSU-23 medium with or without L-carnitine supplementation.
Results: L-carnitine addition had no significant influence on the maturation, cleavage and development to the blastocyst stage versus the control. However, the total cell count of blastocyst from L-carnitine groups were higher (45.0 ± 3.1, 46.4 ± 4.9, 44.6 ± 2.7 for 1.0, 0.5 and 0.25 mg/ml, respectively) than the control (34.5 ± 1.6). Also, zygotes development to the blastocyst stage had no difference, but the  total cell count of blastocyst derived from L-carnitine groups (46.5 ± 12.7, 42.2 ± 14.2, 45.5 ± 9.1 for 1.0, 0.5 1nd 0.25 mg/ml, respectively) were higher than the control (38.6 ± 13.2).
Conclusion: The results demonstrated the efficiency of the procedures used in the maturation, fertilization and culture of porcine oocytes and early- stage embryos. That, L-carnitine supplementation regardless of the concentrations used in the maturation and culture media improved the developmental potential of blastocyst stage embryos as indicated by a higher total cell count (improved cell activity).

 

Download PDF: