Expression of enzyme P450C17 encoding gene CYP17 in the brain of Labeo rohita (HAM.)

Brain is a steroidogenic organ which produces steroids. Our study has been focused to analyze the gene expression in the brain of an Indian Major Carp (IMC), Labeo rohita. This is the first attempt to report the gene expression of CYP17 which codes for the steroidogenic enzyme P450c17 in L. rohita. The brain samples were collected and Total RNA was isolated. The isolated RNA was reverse transcribed into cDNA using RT-PCR. The synthesized cDNA was then added with the gene specific primer and subjected to PCR. Two bands at 402bp and 550bp were obtained which confirms the CYP17 gene expression. The PCR product was sequenced. The obtained sequences of L. rohita were multiple aligned with mammals and with fishes. Phylogram were also plotted to know the relationship, similarity and evolutionary relationship of L. rohita with other organisms which shows that L. rohita is far distantly placed in the evolutionary tree.