Expression and purification of envelop capsid proteins of foot and mouth disease virus type o isolated in vietnamin baculovirus system for making virus-like particle

Nguyen Phuong Hoa, Nguyen Hoang Duong, Tran Thi Kim Dzung, Le Thi Hong Minh, Vu Thi Thu Huyen, Nguyen Mai Anh, Nguyen Thi Kim Cuc and Pham Viet Cuong

Virus-like particles are composed of envelop capsid proteins without containing genetic materials that mimic overall structure of native virions. They are able to effectively elicit both humoral and cell-based immune response but unable to self-replication. This study was designed to construct recombinant baculovirus co-expression of VP1-2A-VP3 and VP0 coding sequences of foot and mouth disease virus (FMDV) type O strain FMDV/HN/2013 under the control of p10 and polyhedrin promotors in baculovirus system. Expressed 2A protease could autoclave VP1-2A-VP3 into VP1 and VP3 showed in western blot. The recombinant envelop capsid proteins VP0, VP1 and VP3 could self-assemble into virus-like particles (VLP) in Sf9 cells with the size similar to authentic FMDV particles.

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           Prof. Dr. Bilal BİLGİN

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   Vol. 07, Issue 02, February 2017



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