Expression and purification of envelop capsid proteins of foot and mouth disease virus type o isolated in vietnamin baculovirus system for making virus-like particle

Author: 
Nguyen Phuong Hoa, Nguyen Hoang Duong, Tran Thi Kim Dzung, Le Thi Hong Minh, Vu Thi Thu Huyen, Nguyen Mai Anh, Nguyen Thi Kim Cuc and Pham Viet Cuong
Abstract: 

Virus-like particles are composed of envelop capsid proteins without containing genetic materials that mimic overall structure of native virions. They are able to effectively elicit both humoral and cell-based immune response but unable to self-replication. This study was designed to construct recombinant baculovirus co-expression of VP1-2A-VP3 and VP0 coding sequences of foot and mouth disease virus (FMDV) type O strain FMDV/HN/2013 under the control of p10 and polyhedrin promotors in baculovirus system. Expressed 2A protease could autoclave VP1-2A-VP3 into VP1 and VP3 showed in western blot. The recombinant envelop capsid proteins VP0, VP1 and VP3 could self-assemble into virus-like particles (VLP) in Sf9 cells with the size similar to authentic FMDV particles.

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   Vol. 07, Issue 02, February 2017

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