Evaluation of leaves´ essential oil of myrcia guianensis (aubl.) dc. from legal amazon: chemical, antibacterial andtoxicity analysis

Myrcia guianensis (Aubl.) DC., a species belonging to the Myrtaceae family, is an endemic plant in tropical areas used by communities in Legal Amazon to treat diarrhea and uterine infections. Although about its medicinal use, there is no description in the literature of biological activities nor determination of components of the essential oil of this species from that Cerrado biome. The present study aimed to perform chemical, biological and toxicity analysis of the essential oil (EO) of the leaves of Myrcia guianensis harvested from Legal Amazon. The EO was obtained by hydrodistillation process and analyzed through GC-MS. The yield calculation resulted in 1.34% and the major compounds were (E-) caryophyllene (37.43%), terpinolene (14.82%), β-bisabolene (6.07%), highlighting that the OE showed a mixture of mono and sesquiterpenes hydrocarbon and oxygenated. The evaluation of the major compounds on PASS, Osiris, PROTOX and Swiss ADME, showed great potential of antimicrobial activity and low toxicity. The essential oil was evaluated against American Type Culture Collection (ATCC) and clinical isolated bacteria, Gram-positive and Gram-negative, and it presented 2mg/mL for MIC against Staphylococcus aureus (ATCC 6538) and MRSA, decreasing effectively cell viability and metabolism. The inhibition of biofilm production was observed against Escherichia coli (ATCC 042). The essential oil of the leaves of Myrcia guianensis has low toxicity against Tenebrio molitor larvae and it promoted a great reduction on Tenebrio molitor larvae hemolymph bacterial load. It was concluded that essential oil of the leaves of Myrcia guianensis has bioprospecting potential for the development of pharmaceutical products.