Estimation of sliver nanoparticles toxicity on human gut micro flora

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International Journal of Development Research

Estimation of sliver nanoparticles toxicity on human gut micro flora

Abstract: 

We synthesized silver nanoparticles (AgNPs) by using the biological material, the extract of Allium sativum (Garlic) and chemically synthesized AgNPs by using silver nitrate. The reduction of pure Ag+ ions was monitored by measuring the UV-Vis spectrum of the reaction medium at 2 hours after diluting a small aliquot of the sample into distilled water. Toxicity of AgNPs was tested using toxtrak test in which, fresh overnight broths of Bacillus subtilis and resazurin dye was used to calculate percentage inhibition (PI). The PI is a relative measure only and since there is toxic substances that increase respiration, to give result in a negative number. The PI of both chemically and biologically synthesized AgNPs was compared in order to evaluate toxic effect value. The toxic effect value PI of chemically synthesized AgNPs is much greater (85.45%) than the biologically synthesized AgNPs from Garlic (46.35%). These observation shows that the bacteria Bacillus subtilis killed by chemically synthesized AgNPs are more as compare to biologically synthesized AgNPs.

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