Erythrocyte alterations of the laying hens subjected to thermal stress
International Journal of Development Research
Erythrocyte alterations of the laying hens subjected to thermal stress
Received 06th March, 2021; Received in revised form 21st April, 2021; Accepted 08th May, 2021; Published online 26th June, 2021
Copyright © 2021, João Rogério Centenaro et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The aim of this study was to identify erythrocyte changes resulting from heat stress in relation to different times of blood collection throughout the day. The experiment was carried out with 39 Rhode Island Red hens, in the initial laying phase, receiving standardized ad libitum feeding. The birds were placed in an environment of 20m2, with controlled temperature, divided into 2 distinct groups: Control Group with 12 animals at 16°C and Experimental Group with 27 animals at 30°C. Venipuncture took place at 7:00 am, 11:00 am and 3:00 pm. Blood was stored in an EDTA tube, aiming at cell preservation for further analysis. Total red blood cell count, hematimetry and erythroid morphometry were performed. After evaluating the erythroid and hematimetric parameters of the 39 birds, a negative correlation of heat stress was observed when comparing the different times of blood collection throughout the day. For morphometric correlation, there was a significant difference (P>0.01) at 3:00 pm. When evaluating the results between the times of blood collection from the same animal, it was observed that 55.5% reduced the hematocrit, while the total red blood cell count was reduced by 51.8%. It is essential that there is a systematization of time in the collection of blood from laying hens.
