Electrophoretic analysis of expression of trypsin inhibitors during chickpea (cicier arietinum L.) seed development

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Author: 
Sudhir R. Bale
Abstract: 

Chickpea (Cicer arietinum L.) is one of the important pulse crops in India which provides high quality proteins for vegetarians. India shares 75% of the world’s chickpea supply. Investigation on accumulation of protease inhibitors during chickpea seed development was carried out. A total of eight genotypes were investigated for expression of trypsin inhibitors during seed development by using polyacrylamide gel-X-ray film contact print method. Electrophoretic profiles of trypsin inhibitors (TI) revealed the results in which a total of 6 isoinhibitors of trypsin were detected in the mature seeds of chickpea. All the eight genotypes exhibited no expression of trypsin inhibitors at 15 days after flowering (DAF). Six genotypes (ICCL-86111, Digvijay, ICC-4958, Chafa, WR-315 and PG-9758) showed no expression of trypsin inhibitors up to 30 DAF. Two genotypes (ICCV-10 and 3137) started expressing trypsin inhibitors around 30 DAF.

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