Efficient protocol for micropropagation of tylophora indica (burm f.) merill an endangered medicinal plant through nodal explants
International Journal of Development Research
Efficient protocol for micropropagation of tylophora indica (burm f.) merill an endangered medicinal plant through nodal explants
Received 16th July, 2017; Received in revised form 20th August, 2017; Accepted 27th September, 2017; Published online 10th October, 2017
Copyright ©2017, Prashanti and Rajeev. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The present study deals with an efficient in vitro propagation of an endangered medicinally important Tylophora indica (Burm f.) Merill well known in traditional medicine for the treatment of asthma, bronchitis, whooping cough and dysentery. Micropropagation of T.indica was carried out using various combinations and concentrations of growth hormones among which best result was observed with Adenine sulphate (2.0mg/l) in combination with BAP(1.0mg/l).A callus free multiple shoot formation was observed with the above combination. Rooting from the in vitro grown shoots were induced from ½ strength MS medium supplemented with IBA (1.0mg/l).The plantlets obtained were successfully acclimatized and transferred under field condition. The present protocol employed in this study can be used for mass propagation of T.indica to meet the pharmaceutical demands for commercial purpose.
