Efficacy of casein phosphopeptide - amorphous calcium phosphate in combating sports drinks dental enamel erosion – an invitro study
International Journal of Development Research
Efficacy of casein phosphopeptide - amorphous calcium phosphate in combating sports drinks dental enamel erosion – an invitro study
Received 22nd July, 2017; Received in revised form 23rd August, 2017; Accepted 27th September, 2017; Published online 10th October, 2017
Copyright ©2017, Swetha et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Background:- Sports drinks are becoming increasingly popular as well as being encouraged with regular exercise. pH of these drinks are less than normal pH and found to be acidic. These acidic beverages are thought to increase potential for dental erosion. Casein Phosphopeptide - Amorphous Calcium Phosphate CPP-ACP complex helps to release Calcium, Phosphate ions to form apatite crystals. In acidic conditions, these calcium, phosphate ions are released from crystal complex and facilitate to reduce the extent of demineralization. This concept forms the basis for the study. Aim:- To assess the efficacy of CPP-ACP in combating dental erosion caused by sports drinks. Settings and design:- This study is an invitro clinical study. Material and Methods:- Extracted premolar teeth of human dentition free of dental caries and hypo calcification were selected. Teeth were sectioned to obtain 3 enamel sections from each tooth to obtain 15 sections. Specimens were immersed in Sports drink (Red bull) Procedure (A),Sportsdrink (Redbull) +CPP-ACP Procedure (B) and Control Procedure (C) for 48 hours,24 hours,12 hours,6 hours,3 hours. pH Values before and after procedure were recorded and analyzed. After completion of immersion time, Tooth specimens were sectioned using Hard tissue Microtome; viewed for extent of demineralization under Polarized light microscope. Statistical analysis:- One way ANOVA was used to compare the difference in mean of demineralization among the groups. Results:- Specimens subjected to Sports drink(A),showed wider areas of enamel demineralization. One way analysis of variance (ANOVA) between the groups shows that F value is 4.977, df=2, at 5% level of significance. Sports drink+ CPP-ACP (B)(193+ 14) showed decreased amount of demineralization when compared to Sports drink (A)(264+19). Conclusion:- CPP-ACP has the ability to reduce demineralization caused by sports drinks. It facilitates Protective action of Loss of mineral content of teeth caused by Sports drink erosion.
