The effectiveness of flavonoid and terpenoid isolate sarang semut (myrmecodia pendens merr and perry) against phorphyromonas gingivalis atcc 33277
International Journal of Development Research
The effectiveness of flavonoid and terpenoid isolate sarang semut (myrmecodia pendens merr and perry) against phorphyromonas gingivalis atcc 33277
Received 09th April, 2017; Received in revised form 24th May, 2017; Accepted 26th June, 2017; Published online 22nd July, 2017
Copyright© 2017, Zulfan M. Alibasyah et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Ethyl acetate fraction of sarang semut plants (Myrmecodia pendens Merr and Perry) were reportedto contain flavonoids and terpenoids which have antibacterial activity against dental cariesbacteria. The aim of this study is to isolate flavonoids and terpenoids from M. pendens tubers and to test their effectiveness against Porphyromonas gingivalis ATCC 33277bacteria which are the main cause of periodentitis. Flavonoid and terpenoid were isolated using chromatographic methods. Characterization of flavonoids and terpenoids is done using UV, IR, NMR, and MS spectroscopy. Minimum inhibitory concentration (MIC) and minimum bacterioside concentration (MBC) were tested using micro dilution method. The result showed that flavonoids and terpenoids isolated from M. pendans effectively inhibited the growth of these periodontitis phatogens at a minimum inhibitory concentration (MIC) of19.57 and 39.06 µg/mL respectively. These compounds also killed the periodontitis phatogens at a minimum bactericidal concentration (MBC) of 312.5 and 625 µg /mL respectively. Our study concluded that flavonoids and terpenoids isolated from M. pendens were effective antibacterial agents against P. Gingivalis ATCC 33277.
