Effect of cigarette smokes density on histophatologis testis of rats

The aim of this study was to know the effect of cigarette smokes to the diameter of seminiferous tubulus testis and the number of Leydig cell of rats exposed to the cigarettes smoke. Some constituents of cigarette smoke was nitrogen, amoniac, CO*,CO2, NO* , NO2*, H2S, Tar, nicotin and metal would influence reproductive system. Twenty four adult male rats were divided to four groups i.e : one group for control (C) and three groups for treatment by cigarette smoke of 38 mV cigarette smokes (T1), 63 mV cigarette smokes (T2), and 181 mV cigarette smokes (T3). The rats in the each treatment group were exposed to the cigarette smoke for 30 days (15 minutes every day). The rats in the control group were kept in the fresh room air. The result of this treatment showed that the diameter of seminiferous tubulus testis of rats between control group and treatment groups were significantly different. (p < 0.01), but there was no significant difference in the diameter of seminiferous tubulus testis rats between treatment groups by cigarettes smoke.The mean average diameter of seminiferous tubulus of control group 54.348 ± 16.60 µm; 40.63 ± 5.44 µm T1 group; 37.44 ± 6.02 µm for T2 group and 33.57 ± 6.70 µm for T3 group. The mean number of Leydig cell were 33.57 ± 4.43 cells for control group (C); 24.5 ± 2.95 cells for T1 group; 22 ± 8.63 cells for T2 group and 19 ± 1.19 cells for T3 group. The number of Leydig cell for control group and treatment groups were respectively different. (P<0.01), but there was no significant difference of the number of Leydig cell between treatment groups. The result of this study indicates that cigarette smokes influenced the histological structure of rats testis.